双向冲水法在无肝素血液透析中的应用效果

目的探讨双向冲水法用于无肝素血液透析中对患者透析器及静脉壶凝血程度、透析时间的影响。方法选取2018年12月至2019年12月于医院行无肝素血液透析的62例患者,按随机数字表法分为两组,各31例。对照组予以传统冲水法,观察组予以双向冲水法,比较两组透析器及静脉壶凝血程度、透析时间。结果两组透析器凝血程度比较,差异无统计学意义(P>0.05);观察组静脉壶凝血程度低于对照组,差异有统计学意义(P<0.05);观察组透析时间长于对照组,差异有统计学意义(P<0.05)。结论双向冲水法可降低无肝素血液透析患者静脉壶凝血程度,延长透析时间,增强透析效果。     

海南省少数民族地区37 482例新生儿遗传代谢病筛查结果分析

目的探讨海南省少数民族地区新生儿多种遗传代谢病(IEM)的发病情况。 方法选择2018年1月1日至2020年1月31日，在海南省少数民族地区出生、监护人同意进行新生儿IEM筛查的37 482例新生儿为研究对象。采用串联质谱法，对新生儿足跟血(滤纸干血斑标本)进行新生儿多种IEM筛查，对结果疑似IEM的部分新生儿，釆集外周血进行基因检测，以确诊其IEM种类、基因突变情况。采用χ²检验，对海南省少数民族地区男性与女性患儿IEM最低发病率进行统计学比较。本研究经海南省人民医院医学伦理委员会批准[审批文号：医伦(2018)第14号]。 结果①海南省少数民族地区上述研究时间内出生的38 586例活产新生儿中，37 482例接受IEM筛查，新生儿IEM筛查覆盖率为97.1%。②37 482例新生儿中，筛查结果疑似IEM的新生儿为89例，其中24例接受基因检测，19例确诊为IEM，24例疑似IEM新生儿的IEM确诊率为79.2%(19/24)。由于仅部分可疑IEM新生儿接受基因检测，故海南省少数民族地区新生儿IEM最低发病率为51/10万(19/37 482)。其中，男性新生儿IEM最低发病率为57/10万(12/21 136)，女性为43/10万(7/16 346)，二者比较，差异无统计学意义(χ²＝0.354、P＝0.552)。③19例确诊IEM新生儿均发生基因突变，其中氨基酸代谢病为5例、有机酸代谢病为5例、尿素循环障碍为2例、脂肪酸β氧化代谢障碍为7例。 结论海南省少数民族地区新生儿IEM发病率较高、种类较多。建议在海南省少数民族地区开展新生儿多种IEM筛查，改善IEM新生儿生活质量。     

新版GMP规范下无菌制剂洗烘灌封联动分装线URS

URS是设备采购的重要技术资料,针对新版GMP规范要求,结合洗烘灌封联动分装线系统及无菌制药工艺特点制定相关URS。     

Mitochondrial DNA control region sequences may differentiate Yanbian Koreans in China from other Asian populations

Abstract

Background: Sequence polymorphisms of mitochondrial DNA (mtDNA) are valuable in forensic medicine and anthropological genetics.

Aim: This study investigated the mtDNA control region sequences in 295 unrelated individuals living in the Yanbian Korean Autonomous Prefecture in the People’s Republic of China.

Subjects and methods: DNA was extracted from blood stained filter papers. Hypervariable regions of the mtDNA control region (HVI and HVII) were amplified and sequenced. The resulting sequences were aligned and compared with the revised Cambridge reference sequence (rCRS).

Results: A total of 182 variations were confirmed. Population comparison showed the significant difference between Yanbian Korean and other included populations.

Conclusion: These results provide useful data for human genetic studies and forensic examinations and demonstrate that the Yanbian Korean population is an endogamous Northeast Asian group.     

Xpert MTB/RIF试验快速诊断结核病的研究进展

<正>结核病是由结核分枝杆菌(Mycobacterium tuberculosis,MTB)感染引起的慢性传染病,仍然是全世界公共卫生面临的重大威胁。据世界卫生组织统计~([1-2]),我国2014年的新发肺结核人数位居全球第三位,是22个全球结核病高负担国家之一。目前肺结核的诊断主要依靠痰涂片抗酸染色,但由于痰涂片对标本的含菌量要求高,导致其诊断结核病的敏感度较低。诊断结核病的金标准是MTB培     

Cesarean Scar Pregnancy: Comparing the Efficacy and Tolerability of Treatment with High-Intensity Focused Ultrasound and Uterine Artery Embolization

The aim of this study was to investigate the clinical efficacy of high-intensity focused ultrasound (HIFU) for the treatment of a cesarean scar pregnancy compared with uterine artery embolization (UAE) and intra-arterial methotrexate infusion combined with uterine curettage. In this retrospective cohort study, 31 patients were treated with HIFU (HIFU group), and 45 patients were treated with UAE (UAE group). We compared the treatment and recovery of the patients, including follow-up. After UAE treatment, serum levels of the β subunit of human chorionic gonadotropin declined significantly on the first day, and the residual lesions disappeared in 3–17 wk. One patient underwent hysterectomy; intrauterine adhesions were found by hysteroscopic examination after 6 mo in 2 patients, whose menstrual function did not return to normal. The remainder of the 42 patients recovered normal menstrual functioning during the 3- to 18-wk follow-up. In the patients who underwent HIFU treatment, serum β-HCG levels did not decline rapidly; serum β-HCG levels increased in many patients and then declined to normal steadily within 2–12 wk. Lesions detached in 3–14 wk in all patients, and menstrual functioning was recovered in 3–9 wk without uterine curettage. Compared with the UAE group, the HIFU group had less pain and fewer complications; the patients in the HIFU group were not hospitalized or anesthetized and had lower costs. HIFU is an efficient, tolerable and non-invasive treatment.     

Involvement of RNA helicase p68 in skin wound healing process in rats

Purpose: RNA helicase p68 plays an important role in organ development and maturation through tuning cell proliferation. However, the character and role of p68 in the whole wound healing process need more study. Methods: First, we characterize expression of p68 in normal rat skin development postnatal. Then, we assayed dynamic change of p68 in rat skin from different stage after injury, and explored the role of p68 in proliferation and migration of three types of wound healing related cells. Results: p68 was down-regulated during skin developmental and maturation process, up-regulated after wound, peaked on day 14 and then significantly decreased. Wound fluid enhanced wound healing related cell proliferation and up-regulated expression of p68. Conversely, reducing p68 expression by RNA interference resulted in significantly slower proliferation and migration. Conclusion: Our results define an important role of RNA helicase p68 in skin wound healing process.     

对我国多媒体健康教育项目发展的思考与启示

系统分析国外多媒体技术在病人健康教育中的应用研究,总结归纳多媒体项目设计特征与应用效果,以期为我国多媒体健康教育项目成功建设提供一定借鉴。     

广东梅州地区性病门诊3824例支原体检测及药敏分析

目的 了解梅州地区性病门诊就诊患者支原体引起泌尿生殖道感染的状况及支原体感染的药敏情况.方法 2008年8月至2011年3月对性病门诊拟诊为尿道炎、阴道炎或宫颈炎的3 824例就诊者,采用珠海迪尔生物工程公司支原体培养、药敏一体化试剂盒进行支原体培养,并对阳性患者进行12种抗生素的药敏试验.结果 共培养出1 103例支原体感染者(阳性率28.24%),解脲支原体(Uu)743株(67.36%),人型支原体(Mh)24株(2.18%),两种支原体混合感染336株(30.46%).对Uu药敏感率最高的药物为交沙霉素,其次为多西环素和美满霉素;对Mh及Uu/Mh两种支原体混合感染药物敏感也呈同样趋势.对Uu耐药最高的是环丙沙星,其次是红霉素和氧氟沙星;对Mh耐药最高的红霉素,其次是阿奇霉素和克拉霉素,对Uu/Mh两种支原体混合感染耐药最高的是红霉素,其次是氧氟沙星和罗红霉素.结论 临床用药应该考虑该地区耐药株的流行情况,最好按照药敏试验结果合理用药.